拟穴青蟹(Scylla paramamosain)六个地理群体肌肉氨基酸和脂肪酸的比较分析     

李永亮  赵欢欢  李荣华  王春琳  母昌考  宋微微  叶央芳 《海洋与湖沼》2019,50(2):465-472

本文对广西钦州(Gq)、福建霞浦(Fx)、浙江温州(Zw)、浙江三门(Zs)、浙江宁海(Zn)、浙江慈溪(Zc)等6个野生群体拟穴青蟹(Scylla paramamosain)肌肉进行了氨基酸及脂肪酸分析。共测定出19种游离氨基酸和26种脂肪酸。氨基酸含量在80.94—191.39mg/g之间, Gq为最高;总脂肪酸含量在7.97—11.38mg/g之间,Zn为最高。主要氨基酸为甘氨酸和精氨酸;主要脂肪酸为C20:5n3(EPA),占总脂肪酸含量的平均值为(22.47±5.84)%。各群体风味氨基酸和多不饱和脂肪酸含量丰富,占总氨基酸比例(TDAA/TFAA)和总脂肪酸比例(PUFA/TFA)分别为(53.62±5.43)%和(59.27±5.29)%。Zs群体的必需氨基酸含量及C22:6n3(DHA)均为最高。主成分分析结果表明:各群体的氨基酸与脂肪酸组成与含量相似性与地理距离分布具有一定的相关性,特别是Gq群体,在氨基酸与脂肪酸的分类中与其他群体区分比较明显。各群体风味氨基酸和多不饱和脂肪酸的聚类分析结果均表明:Zw、Zc与Gq群体较为接近,具有较强的甜味; Fx、Zn与Zs群体较为接近,具有较强的香味及不饱和脂肪酸营养价值。  相似文献   

Biological and granulometric controls on sedimentary organic matter of an intertidal mudflat     

Lawrence M. Mayer  Peter T. Rahaim  William Guerin  Stephen A. Macko  Les Watling  Franz E. Anderson 《Estuarine, Coastal and Shelf Science》1985,20(4):491-503

A variety of measures of organic matter concentration and quality were made on samples collected from the top few mm of intertidal mudflat sediment over the course of a year, in order to assess the relative importance of biological and sedimentological influences on sedimentary organic matter. Winter and summer were times of relatively fine-grained sediment accumulation, caused by biological deposition or stabilization processes and resulting in higher organic matter concentrations. Stable carbon isotope and Br:C ratios indicated a planktonic source of bulk organic matter. Ratios of organic carbon to specific surface area of the sediments were consistent with an organic monolayer coverage of sediment grains. Correction for changing grain size during the year showed no change in the organic concentration per unit surface area, in spite of organic matter inputs by in situ primary production, buildup of heterotroph biomass and mucus coatings, and biodeposition of organic-rich seston. There were also no indications of changes in bulk organic quality, measured as hydrolyzable carbohydrates and amino acids, in response to these biological processes. It is concluded that biological processes on a seasonal time scale affect the bulk organic matter of these sediments via a modulation of grain size rather than creation or decay of organic matter.  相似文献   

Phaeocystis globosa与Phaeocystis antarctica叶绿体psbA基因的比较   总被引：3，自引：0，他引：3       下载免费PDF全文

杨泽民  章群  谢数涛  韩博平  吕颂辉  Hodgkiss 《海洋科学》2005,29(1):24-28

测定了2株球形棕囊藻Phaeocystis globosa P1、P2的psbA基因序列。发现得到的2个序列完全相同。以P2序列对比分析了P．globosa和P．antarctica的psbA基因在DNA序列、氮基酸序列和RNA二级结构上的差异性，发现2种棕囊藻psbA基因DNA序列和氨基酸序列非常保守，无插入／缺失，其核苷酸和氨基酸变异率分别为1．88％和1．13％。与核基因核苷酸的碱基替换不同，psbA基因核苷酸的碱基替换主要发生在密码子的第1位上。且不引起氨基酸的变化，引起氨基酸变化的碱基替换都发生在密码子的第2位和第3位上。在RNA二级结构上两序列的1～4茎环结构完全相同，表现出明显的棕囊藻属的特异性，其它结构区域差异较大。种间差异表现明显。由于psbA基因DNA序列和氨基酸序列非常保守，可能不适宜棕囊藻属的系统发育分析。但其RNA二级结构可能对于棕囊藻的分子分类有一定的参考价值。  相似文献   

斑纹鲟凝集素(CFL)的初步研究 Ⅱ.CFL的分子组成及性质鉴定   总被引：3，自引：0，他引：3  

戴聪杰  王桂忠  李少菁  艾春香 《海洋学报》2006,28(3):98-102

采用生化方法对斑纹鲟(Charybdis feriatus)凝集素进行了初步研究.结果表明,斑纹鲟的血清中有动物红细胞和微生物细胞凝集活性的凝集素(Charybdis feriatuslectin,CFL),其活力表现依赖于Ca2+,并为多种糖所抑制;CFL对40℃以上的温度敏感,其活力在pH6.0~8.0内稳定.经巯基乙醇处理和未经巯基乙醇处理的CFL在SDS-PAGE上均为一条蛋白带,说明斑纹鲟凝集素不含二硫键,且只有一种亚基,分子量为40.8 kDa,CFL氨基酸组成分析表明其富含酸性氨基酸.  相似文献   

Variation of reactivity of particulate and sedimentary organic matter along the Zhujiang River Estuary   总被引：3，自引：0，他引：3  

Chen Jianfang  Jin Haiyan  Yin Kedong  Li Yan 《海洋学报(英文版)》2003,22(4):557-568

To investigate organic matter source and reactivity in the Zhujiang River (Pearl River)Estuary and its adjacent areas, particulate organic carbon (POC), particulate hydrolysable amino acids (PHAA), and Chl a during two cruises in July 1999 and July 2000 were measured. The highest POC and PHAA concentration was observed in the waters with maximum Chl a. The spectra distribution,relative content (dry weight in milligram per gram), PHAA-C% POC and other indicators such as the ratios of amino acids vs. amino sugars (AA/AS) and glucosamine vs. galactosamine (Glum/Gal) suggested that particulate amino acids in the water column and sediments in the Zhujiang River Estuary were mainly derived from biogenic processes rather than transported from terrestrial erosion. In inner estuary where high turbidity was often observable, organic matter was mainly contributed by re-suspension of bottom sediments with revealed zooplankton, microbial reworked characteristics, which suggest that these organic matters were relatively “old“. In the estuarine brackish region, organic matter in water column is mainly contributed by relatively fresh, easily degradable phytoplankton derived organic matter.During physical - biological processes within the eastuary, organic matter derived from phytoplankton was subjected to alteration by zooplankton grazing and bacterial reworking.  相似文献   

Diel fluctuations in dissolved free amino acids and monosaccharides in Chesapeake Bay dinoflagellate blooms   总被引：1，自引：0，他引：1  

K. G. Sellner  E. W. Nealley 《Marine Chemistry》1997,56(3-4)

The accumulation of phytoplankton biomass in recurring summer dinoflagellate blooms of Chesapeake Bay is accompanied by large pools of dissolved organic matter (DOM). Two fractions of the DOM, free amino acids (DFAA) and monosaccharides (MONO), were measured at 3 h intervals in mixed species dinoflagellate blooms (Katodinium rotundatum, Gymnodinium spp.) and related to productivity, biomass and photoperiod. Peak chlorophyll levels for the three blooms were 28, 65 and 938 μg1⁻¹. In general, DFAA and MONO concentrations increased with increasing biomass of bloom-forming species, reaching 203 and 844 μg1⁻¹. MONO appeared to accumulate during the day while there was no consistent pattern for DFAA. The accumulations of DFAA and MONO in blooms indicate that bloom production might stimulate microheterotrophy, thereby enhancing carbon and nutrient cycling in bloom-impacted regions.  相似文献   

东海海域海水中颗粒氨基酸(PAA)的组成与含量的分布调查研究     

李烈英  张星君  纪明侯 《海洋科学集刊》2004,46(46):100-105

海水中的有机物的含量微少，约为0.5~2.0 C mg/L，但其种类复杂。其中以溶解有机物(DOM)为主，而颗粒有机物(POM)所占比例则更少。大洋海水中的POM主要来源于浮游生物等的死骸及其分解碎屑；另外还有海水中受风浪影响所进行的DOM-POM的动力平衡产物。近岸海水还受河流和大气带入的陆源尘影响。POM主要由颗粒氨基酸(PAA)组成。即由Asp、Glu、Thr、Ser、Ala、Gly、Leu、Ile、Val、Met、Cys、Tyr、Lys、His、Arg、Phe、Pro等17种氨基酸组成。它们是食碎动物的主要食物，构成海洋食物链的一环，与该水域生产力有直接联系。纪明侯等(1992)于1983年和1984年首次对青岛胶州湾海水中的颗粒氨基酸的组成和含量的变化及其季节变化进行了调查研究。并且在1982年和1984年前后对渤海湾、黄河口和长江口附近水域同样调查研究了表层海水中PAA的组成与含量分布。他们还于1981年7、8月和1982年7、8月乘“科学一号”考察船进一步研究了长江口外东中国海，包括福建省外海、济州岛附近海域和黑潮流域26°00′-33°00′N、123°00′-129°00′E范围内海域的20余个站位的表层海水和部分站位上不同水深中的PAA的组成和含量。兹将调查研究结果报道如下。  相似文献   

Study of Adsorption Isotherm Curves of Amino Acids on the Kaolinite     

刘效兰  张正斌 《海洋通报(英文版)》2002,4(1):52-60

This paper studies the adsorption isotherm curves of four amino acids (glyucine,aspartic acid,glutamic acid and lysine)and the effects of existences of different concentrations of Cu(Ⅱ) on them.Both the adsorption isotherm of the amino acids on the kaolinite and that when the copper ion Cu(Ⅱ） exists belong to the Langmuir type.The slopes of these adsorption isotherm increase with the increasing concentrations of Cu(Ⅱ） added.It can be explained in terms of formation of the type(Ⅰ） ternary surface complexes in these systems.The regular pattern of the effects of copper ion Cu(Ⅱ) on the adsorption isotherm of amino acids on the kaolinite is identical with that of its effects on the exchange and adsorption percentage E(%)-pH on the kaolinite in the same system.  相似文献   

南海中部和冲绳海槽沉积物中的氨基酸物质   总被引：2，自引：0，他引：2  

郑士龙  唐运千 《海洋学研究》1993,11(2):34-42

本文通过对南海中部和冲绳海槽三个深海沉积物柱状样腐植物质水解氨基酸的研究,试图阐述南海和冲绳海槽沉积物的演化与沉积环境。  相似文献   

Seasonal Changes in Nitrogen, Free Amino Acids, and C/N Ratio in Mediterranean Seagrasses   总被引：2，自引：0，他引：2  

Helmut  Pirc Bernd  Wollenweber 《Marine Ecology》1988,9(2):167-179

Abstract. Seasonal changes in nitrogen, free amino acids, and carbon were investigated in the three Mediterranean seagrasses Posidonia oceanica, Cymodocea nodosa , and Zostera noltii. Leaves, rhizomes, roots, as well as dead plant material were analysed separately. Highest N-concentrations were obtained in the winter months, regardless of species or plant part. In contrast to the other two species, the N-content in Posidonia was higher in the rhizomes than in the leaves.
In the investigated species, marked differences in the free amino acid (FAA) composition were detected between species: Proline, lacking in Posidonia , was the main component in Cymodocea. In Posidonia , FAA decreased from 320umol g^-1 (dry wt) in leaf sheaths to 1.5 umol g^-1 (dry wt) in the leaf tips. The function of proline as a possible storage and/or stress metabolite is discussed.
High C/N values in dead P. oceanica and C. nodosa rhizomes as well as in P. oceanica wrack seem to be related to considerable resistance to decomposition. Low C/N ratios did not increase in detached C. nodosa and Z. noltii leaves, which began to decompose shortly after detachment from the plants.  相似文献